celltrace tm violet cell proliferation kit (Thermo Fisher)
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celltrace tm violet cell proliferation kit
Celltrace Tm Violet Cell Proliferation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltrace+violet+cell+proliferation+kit/celltrace+violet/pmc12281710-105-0-10
Average 90 stars, based on 1 article reviews
Celltrace Tm Violet Cell Proliferation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltrace+violet+cell+proliferation+kit/celltrace+violet/pmc12281710-105-0-10
Average 90 stars, based on 1 article reviews
celltrace tm violet cell proliferation kit - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Transplants foster B cell alloimmunity by relaying extracellular vesicles to follicular dendritic cells"
Article Title: Transplants foster B cell alloimmunity by relaying extracellular vesicles to follicular dendritic cells
Journal: Cell reports
doi: 10.1016/j.celrep.2025.115832
Figure Legend Snippet: (A) Donor H2 Ag in splenic FDCs (inset, arrows) after transplantation of B6 (H2 b ) hearts in BALB/c mice. Original magnification ×200. Scale bars, 20 μm. (B) Quantification with ImageJ of donor H2 Ag spots per FDC network on spleens of BALB/c mice transplanted with B6 hearts. (C) Donor H2 Ag spots per FDC network on BALB/c LNs draining B6 skin allografts assessed using ImageJ. (D) Deep SIM of donor H2 Ag spots in FDCs of a BALB/c LN draining a B6 skin graft. Scale bar, 2 μm. ROI, region of interest. (E and F) 2P-microscopy of FDCs in the spleen of a BALB/c mouse transplanted with a B6 heart releasing RFP-sEVs (E) and a BALB/c LN draining a B6 skin graft releasing RFP-sEVs (F). Arrows: graft-RFP-sEVs on the surface (red) or inside (yellow) of FDCs (green). CellTrace Violet-B cells indicate B cell follicles. FDCs were labeled with AF488-CD21/CD35 Ab. Original magnification ×25. Scale bars, 20 μm. (G) Colocalization by confocal microscopy of donor (B6, IA b + H2K b ) allo-Ags in FDCs of LNs draining CMV Cre/+ LSL -RFP-CD63 B6 skin grafted in BALB/c mice. Scale bar, 10 μm. Mander’s colocalization coefficient of graft-sEVs and donor IA b + H2K b Ab analyzed using Imaris. Each triangle represents one FDC network. In (E) and (F), RFP spots per FDC network z stacks were quantified within 90-μm-thick z stacks using Imaris. Experiments were done on 4 spleens per POD (A and B), 6 graft-dLNs per POD (C), 3 graft-dLNs (D), 4 spleens (E), 4 graft-dLNs (F), and 12 graft-dLNs (G). In (B), (C), (E), and (F), comparisons were made by multiple unpaired two-tailed Student’s test. Error bars, means ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; NS, not significant.
Techniques Used: Transplantation Assay, Microscopy, Labeling, Confocal Microscopy, Two Tailed Test
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In vitro T cell proliferation assay lymph node t cells isolated from c57Bl/6 and Ot-ii transgenic mice were stained with 1 μM celltrace Violet (ctV) cell Proliferation Kit (thermo Fisher scientific, Waltham, Ma) for flow cytometry and incubated at 37 °c for 3 min. the labeled cells were mixed at 1:1 ratio. then, 1 × 105 ctV-labeled mixed cells were co-cultured with 10 μg/ml of aP-eVs, control seVs, or anti-mouse cD3/cD28 beads (thermo Fisher scientific, Waltham, Ma) for three days. subsequently, Proliferation Assay:Article Title: Selective expansion and differentiation of antigen-specific CD4 + T-helper cells by engineered extracellular vesicles. Article Snippet: Following this, the grid was blotted to remove excess stain and left to air-dry at ambient temperature. the negatively stained eV samples were then visualized using a JeOl JeM-1400Flash electron microscope (akishima, Japan) operating at an accelerating voltage of 100 kV. .. 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